LOCUS       40924_45123        4186 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUC18VcluxOR DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Kasai S.
  TITLE     Freshwater bioluminescence in Vibrio albensis (Vibrio cholerae 
            biovar albensis) NCIMB 41 is caused by a two-nucleotide deletion in 
            luxO
  JOURNAL   J. Biochem. 139 (3), 471-482 (2006)
  PUBMED    16567412
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Kasai S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2005) Contact:Sabu Kasai Graduate School of 
            Engineering, Osaka City University, Department of Applied Chemistry 
            and Bioapplied Chemistry; Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 
            558-8585, Japan
REFERENCE   3  (bases 1 to 4186)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4186)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2006"; volume: "139"; issue: "3"; pages: "471-482"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-2005) Contact:Sabu Kasai Graduate School of Engineering, 
            Osaka City University, Department of Applied Chemistry and 
            Bioapplied Chemistry; Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 
            558-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2259..3783
                     /strain="NCIMB 41"
                     /mol_type="other DNA"
                     /note="biovar:albensis; synonym: Vibrio cholerae bv.
                     albensis"
                     /db_xref="taxon:140100"
                     /organism="Vibrio albensis"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2412..3779
                     /codon_start=1
                     /gene="luxO"
                     /product="LuxO"
                     /label=luxO
                     /note="derived from Vibrio cholerae biovar albensis lux0
                     gene, deposited in Acc# AB114423, a two nucleotide deletion
                     is recoverd"
                     /protein_id="BAE87124.1"
                     /translation="MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLIL
                     LDLRLPDMTGMDVLYAVKEKSPDVPIVFMTAHGSIDTAVEAMRHGTQDFLIKPCEADRL
                     RVTVNNAIRKASKLKNDVDNKNQNYQGFIGSSQTMQAVSRTIDSAASSKASIFITGESG
                     TGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEA
                     ADGGTLFLDELCEMDLDLQTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQ
                     EGRFREDLYYRLYVIPLHLPPLRARGDDVIEIAYSLLGFMSKEEGKDFVRLSAEVVERF
                     RQYEWPGNVRQLQNVLRNVVVLNEGREITLDMLPPPLNQMSVPINRALPLAHENKVSVH
                     EIFPLWMTEKQAIEQAIEACDGNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER"
     gene            2412..3779
                     /gene="luxO"
                     /label=luxO
     primer_bind     complement(3792..3808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattcgag ctcggtaccc ggggatcctc tagatgtgtg atgtttggca
     2281 tcatttatca agaaatttta attcaaattt gcaaaatgca attccaaatg caattattac
     2341 agcaaaatgc aaaataatat ggctaggcta tgcaacataa tcaatctttg caaaaaacaa
     2401 aatatgtcct catggtagaa gacacggcct cggtggcggc gctgtatcgt tcttacctca
     2461 cgccgctgga tattgatatc aatatcgtgg gtaccggacg cgatgccatc gagagtattg
     2521 gccgtcgcga gccggactta atcctgctgg atttacgttt gccagatatg actgggatgg
     2581 acgtactcta tgcagtgaaa gagaaatcac cggatgtgcc tatcgtgttt atgaccgctc
     2641 atggttcgat tgatacggcg gttgaagcca tgcgtcatgg tacgcaagac tttttgatca
     2701 agccgtgtga agcggaccga ctgcgggtca ctgtgaataa tgcgattcgc aaagcctcga
     2761 aactcaaaaa cgatgttgat aataaaaatc aaaactatca aggctttatt ggtagcagtc
     2821 aaaccatgca ggcggtgtcc cgcaccattg actctgcggc gagcagcaaa gccagtattt
     2881 ttattaccgg cgaaagtggt accggtaaag aagtgtgcgc ggaagcgatt catgccgcga
     2941 gcaagcgcgg agataagcca tttatcgcca tcaactgcgc ggccattccg aaagatctga
     3001 ttgaaagtga gttgtttggt catgttaaag gggcttttac cggagcggcg actgagcgtc
     3061 aaggcgcggc agaagcggct gatgggggaa ccctctttct ggatgaattg tgcgaaatgg
     3121 atctggatct gcagaccaaa ctgctgcgct tcatccagac aggaacattc caaaaagtcg
     3181 gctcttccaa aatgaaaagc gtggatgtgc gctttgtgtg tgcaacgaac cgcgatccgt
     3241 ggaaggaagt gcaagaagga cgttttcgtg aagacctgta ctaccgcttg tatgtgatcc
     3301 cgctgcattt accgccattg cgcgcgcgtg gtgatgatgt gatcgagatt gcttattcac
     3361 tgcttggctt tatgtccaag gaagagggca aagattttgt ccgtttgtcg gctgaagtgg
     3421 tggagcgttt tcgtcaatac gagtggccgg gcaacgtgcg tcaattgcaa aacgttttgc
     3481 gcaacgtggt cgtgctcaat gaaggtcgtg aaatcaccct agatatgctg cctcctcctc
     3541 ttaatcaaat gtccgtgccg atcaatcggg ctttaccgct tgcgcatgag aataaagtat
     3601 ccgtgcatga gatttttccg ctgtggatga ccgaaaaaca agccattgag caagccatcg
     3661 aagcgtgtga tggtaacatt cctcgcgccg cgacctatct ggatgtcagc ccgtcaacca
     3721 tctatcgcaa gctgcaaact tggaatgaaa aagtgaaaga aaaagagaag gaacggtaag
     3781 cgaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3841 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3901 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     3961 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     4021 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4081 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4141 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
